Hydrospace PNS HomeGro

PNS HomeGro™ is our black-and-white label, brown-box bulk package (finished product treats 8,000+ gallons!). This unique kit allows users to cultivate larger volumes of PNS bacteria economically using commonplace chemicals and implements. It is also a more environmentally sustainable option, as it significantly reduces shipping/packaging waste.

Each PNS HomeGro™ kit includes one of three species (your choice):

• Rhodopseudomonas palustris (Freshwater or marine, known coral symbiont)
• Rhodospirillum rubrum (Freshwater or marine, excellent "carbon dosing" exporter)
• Rhodobacter capsulatus (Primarily freshwater, known plant symbiont) 

While each of these species presents its own distinct advantages in aquaria, ponds and hydroponic systems, they may be used in tandem for enhanced efficacy. 

Each ingredient in the culture medium mix is individually measured per package (to the hundredth of a gram) to ensure excellent uniformity. Therefore, the user should be able to consistently acheive satisfactory results provided that proper sanitation/biosecurity practices are followed. In addition to sterilizing all implements as described below, wash hands/gloves thoughly with antibacterial soap and apply a hand sanitizer. Avoid working in drafty and/or dusty areas.

The incubation temperature should range between 25-30°C/77-86°F (Rhodopseudomonas and Rhodospirillum tolerate temperatures slightly above this range). Note that the light source may add some heat to the culture vessel.

Pour 1 cap household bleach into culture vessel (use vessel cap). Pour 1 cup hot tap water into vessel. Recap vessel and shake. Uncap vessel and completely pour out bleach water, shaking out last drops (do not rinse). Recap immediately.

Bring 2.5 gallons distilled water to boil in pot. Sterilize implements (stirring spoon, thermometer and funnel) with hydrogen peroxide (preferably by spraying) and place on a sterile surface. Add growth medium and stir well. Cut heat and cool pot to 74°C/165°F. Pour into culture vessel through funnel (keep cap on sterile surface). Recap immediately.

Cool vessel to room temperature. Remove cap and carefully pour inoculum into vessel. Recap with sterilized spigot. Place vessel under continuous moderate (~5,000 lumens) white light. Allow 30 days incubation. Shake vessel just before each use.